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Myo-Inositol Esters of Indole-3-acetic Acid Are Endogenous Components of Zea mays L. Shoot Tissue ¹

Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan 48824-1312

Indole-3-acetyl-myo-inositol esters have been demonstrated to be endogenous components of etiolated Zea mays shoots tissue. This was accomplished by comparison of the putative compounds with authentic, synthetic esters. The properties compared were liquid and gas-liquid chromatographic retention times and the 70-ev mass spectral fragmentation pattern of the pentaacetyl derivative. The amount of indole-3-acetyl-myo-inositol esters in the shoots was determined to be 74 nanomoles per kilogram fresh weight as measured by isotope dilution, accounting for 19% of the ester indole-3-acetic acid of the shoot. This work is the first characterization of an ester conjugate of indole-3-acetic acid from vegetative shoot tissue using multiple chromatographic properties and mass spectral identification. The kernel and the seedling shoot both contain indole-3-acetyl-myo-inositol esters, and these esters comprise approximately the same percentage of the total ester content of the kernel and of the shoot.

¹ Supported by grants from the Metabolic Biology Section of the National Science Foundation, PCM 8204017, ORD 30668, and the Life Science Section of the National Aeronautics and Space Administration, NAGW 97, ORD 25796 to R. S. Bandurski. Mass spectral data were obtained from the Michigan State University Mass Spectrometry Facility supported by Grant RR-00480 from the Biotechnology Resources Branch, Division of Research Resources, National Institutes of Health. This is Journal Article No. 10863 from the Michigan Agricultural Experiment Station.

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