Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 74:170-175 (1984)
© 1984 American Society of Plant Biologists

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Articles

Temperature Dependence of Energy-Transducing Functions and Inhibitor Sensitivity in Chloroplasts 1

Jaap J. Schuurmans, Enno C. I. Veerman, Jan A. Francke, José M. G. Torres-Pereira2 and Ruud Kraayenhof

Plant Physiology Department, Vrije Universiteit, De Boelelaan 1087, 1081 HV The Netherlands, Biological Laboratory, Vrije Universiteit, De Boelelaan 1087, 1081 HV The Netherlands

A comparative analysis of the temperature dependence of energy-transducing reactions in spinach (Spinacia oleracea) chloroplasts and their sensitivity for uncouplers and energy-transfer inhibitors at different temperatures is presented. Arrhenius plots reveal two groups of transitions, around 19°C and around 12°C. Activities that show transitions around 19°C include linear electron flow from water to ferricyanide, its coupled photophosphorylation, the dark-release of the fluorescent probe atebrin, and the slow component of the 515 nm (carotenoid) absorbance decay after a flash. The transitions around 12°C are observed with pyocyanine-mediated cyclic photophosphorylation, light- and dithioerythritol-activated ATP hydrolysis, the dark-release of protons, and the fast 515 nm decay component. It is suggested that both groups of temperature transitions are determined by proton displacements in different domains of the exposed thylakoid membranes. The effects of various uncouplers and an energy-transfer inhibitor are temperature dependent. Some uncouplers also show a different relative inhibition of proton uptake and ATP synthesis at lower temperatures. The efficiency of energy transduction (ATP/e2) varied with temperature and was optimal around 10°C.

2 On leave from the Plant Physiology Laboratory, Instituto Universitário de Trás-os-Montes e Alto Douro, P.O. Box 202, 5001 Vila Real, Portugal, on a fellowship from the Netherlands Government Fellowships Programme.

1 Supported in part by the Foundation for Biophysics with financial aid from the Netherlands Organization for the Advancement of Pure Research (ZWO).






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Copyright © 1984 by the American Society of Plant Biologists