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Plant Physiology 73:233-237 (1983)
© 1983 American Society of Plant Biologists

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Development of a Radioimmunoassay for the Soybean Phytoalexin Glyceollin I 1

Peter Moesta2, Michael G. Hahn3 and Hans Grisebach4

Biologisches Institut II der Universität, Schänzlestrasse 1, D-7800 Freiburg i.Br., Federal Republic of Germany

A radioimmunoassay for glyceollin I, the major phytoalexin produced by soybean (Glycine max [L.] Merr.), has been developed. Antibodies were raised in rabbits against a glyceollin I-bovine serum albumin conjugate. The antisera were used to establish a radioimmunoassay for glyceollin I using [125I]glyceollin I as the tracer. A logit plot of a standard concentration series yielded a straight line in the range of 1 to 100 picomoles (0.34-34 nanograms) of glyceollin I. The structurally related pterocarpan phytoalexins, glyceollins II and III, glyceollidin II and glycinol, which also accumulate in infected soybean tissue, show a low cross-reactivity in the radioimmunoassay (0.5-5% at 50% displacement of the tracer). Two related isoflavones present constitutively in soybean tissue, daidzein and genistein, have cross-reactivities of less than 0.84% and 1.1%, respectively. The radioimmunoassay permitted the quantitative determination of glyceollin I in 15-micrometer microtome sections of soybean hypocotyl tissue infected with zoospores of Phytophthora megasperma f. sp. glycinea.

2 Present address: Department of Chemistry, University of California, Los Angeles, CA 90024.

3 Supported by a Research Fellowship from the Alexander von Humboldt-Stiftung.

4 To whom correspondence should be addressed.

1 Supported by Deutsche Forschungsgemeinschaft (SFB 46), Fonds der Chemischen Industrie, BASF (Ludwigshafen).






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ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1983 by the American Society of Plant Biologists