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Plant Physiology 73:94-99 (1983)
© 1983 American Society of Plant Biologists

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Articles

Terpenoid Metabolism in Plastids 1

Isolation and Biochemical Characteristics of Capsicum annuum Chromoplasts

Bilal Camara, Françoise Bardat, Odette Dogbo, Judy Brangeon2 and René Monéger

Laboratoire de Régulations Métaboliques et Différenciation des Plastes, Université Pierre et Marie Curie (Paris 6), 75230 Paris Cédex 05, France

A technique for the isolation and the purification of Capsicum annum L. var. Yolo Wonder chromoplasts is described. The degree of purity of the isolated chromoplasts is greatly improved by the absence of MgCl2 in the extraction medium and in the gradient purification system, as shown by electron micrographs and the near absence of antimycin-insensitive NADH:cytochrome c reductase activity and succinate:cytochrome c reductase activity. Furthermore, phosphatidylserine was absent and the phosphatidylethanolamine content was reduced by a factor of 5 in these preparations, which were active in the synthesis of galactolipids, prenylquinones, and carotenoids.

2 Present address: Laboratoire de Structure et Métabolisme des Plantes, Bât. 490, F. 91405 Orsay, France.

1 Supported by grants from the Direction de la Recherche Universitaire and from the Centre National de la Recherche Scientifique (AI 03.1269).






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Copyright © 1983 by the American Society of Plant Biologists