Plant Physiol.
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Plant Physiology 73:79-81 (1983)
© 1983 American Society of Plant Biologists

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Articles

Separation of Monovinyl and Divinyl Protochlorophyllides and Chlorophyllides from Etiolated and Phototransformed Cucumber Cotyledons 1

Claire M. Hanamoto and Paul A. Castelfranco2

Department of Botany, University of California, Davis, California 95616

A method was developed to separate the monovinyl and divinyl forms of protochlorophyllide and chlorophyllide by high pressure liquid chromatography using a silicic acid column coated with dodecyl residues and a moving phase containing the lipophilic cation, tetrabutyl ammonium. The solvent was 70% methyl alcohol containing varying amounts of methyl ethyl ketone. The separation was carried out at 0°C. This method was used to test and confirm a previous report that, in cucumber cotyledons, divinyl protochlorophyllide is phototransformed to give divinyl chlorophyllide, which is biologically unstable and disappears rapidly in the dark.

2 Author to whom correspondence should be addressed.

1 Supported by National Science Foundation grant PCM 81-08538.






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Copyright © 1983 by the American Society of Plant Biologists