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Plant Physiology 72:754-758 (1983)
© 1983 American Society of Plant Biologists

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Articles

Biosynthesis of Arabinogalactan-Protein in Lolium multiflorum (Ryegrass) Endosperm Cells 1

III. Subcellular Distribution of Prolyl Hydroxylase

Peter B. Cohen, Angelo Schibeci and Geoffrey B. Fincher

Department of Biochemistry, La Trobe University, Bundoora, Victoria, 3083, Australia

The peptidyl prolyl hydroxylase responsible for the formation of hydroxyproline during arabinogalactan-protein biosynthesis in Lolium multiflorum (ryegrass) endosperm cells is a membrane-associated enzyme which will catalyze the hydroxylation of poly(L-proline) in the presence of oxygen, {alpha}-ketoglutarate, ferrous ion, and ascorbate. The Km for poly(L-proline) (8000 molecular weight) is 40 micromolar. The enzyme will also hydroxylate the protocollagen analog (Pro-Pro-Gly)5·4H2O.

Fractionation of membranes from protoplast lysates on a discontinuous sucrose/sorbitol density gradient, followed by centrifugation on a linear sucrose gradient in the presence of Mg2+, leads to a clear separation of a number of membrane components. The membrane components have been tentatively identified using marker enzymes and assayed for peptidyl prolyl hydroxylase. It is concluded that the ryegrass prolyl hydroxylase is enriched in Golgi-derived membranes, but that significant amounts are also located in other subcellular fractions, including the rough endoplasmic reticulum.

1 Supported by a grant from the Australian Research Grants Scheme. P. B. C. acknowledges receipt of a La Trobe University Research Scholarship.




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R. Hieta and J. Myllyharju
Cloning and Characterization of a Low Molecular Weight Prolyl 4-Hydroxylase from Arabidopsis thaliana. EFFECTIVE HYDROXYLATION OF PROLINE-RICH, COLLAGEN-LIKE, AND HYPOXIA-INDUCIBLE TRANSCRIPTION FACTOR alpha -LIKE PEPTIDES
J. Biol. Chem., June 21, 2002; 277(26): 23965 - 23971.
[Abstract] [Full Text] [PDF]


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Copyright © 1983 by the American Society of Plant Biologists