Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 72:186-189 (1983)
© 1983 American Society of Plant Biologists

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Articles

Changes of Cyanide Content and Linamarase Activity in Wounded Cassava Roots 1

Mineo Kojima, Norio Iwatsuki, Emma S. Data, Cynthia Dolores V. Villegas and Ikuzo Uritani

Faculty of Agriculture, Nagoya University, Chikusa, Nagoya 464, Japan, Philippine Root Crop Research and Training Center (PRCRTC), Visayas State College of Agriculture (ViSCA), Baybay, Leyte, Philippines

When cassava (Manihot esculenta Crantz) root was cut into blocks and incubated under laboratory conditions, the blocks showed more widespread and more even symptoms of physiological deterioration than those under natural conditions. Thus, the tissue block system has potential for biochemical studies of natural deterioration of cassava root. The changes in cyanide content and linamarase (linamarin beta-D-glucoside glucohydrolase; EC 3.2.1.21) activity in various tissues during physiological deterioration were investigated. Total cyanide content increased in all parts of block tissue after 3-day incubation. The degree of increase in cyanide was most pronounced in white parenchymal tissue, 2 to 3 millimeters thick, next to the cortex (A-part tissue), where no physiological symptoms appeared. On the other hand, linamarase activity was decreased in all parts of block tissue after a 3-day incubation. A time course analysis of A-part tissue indicated a clear reciprocal relationship between changes in total cyanide and linamarase activity; total cyanide increased, while linamarase activity decreased. Free cyanide constituted a very small portion of the total cyanide and did not change markedly.

1 This work was supported in part by grants (Project No. 56041028 and 57041021) of the 1981 and 1982 Overseas Scientific Investigation, the Ministry of Education, Science, and Culture, Japan and the Joint Scientific Cooperation Program between the National Science and Technology Authority, Philippines and Japan Society for the Promotion of Science.






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Copyright © 1983 by the American Society of Plant Biologists