Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 71:496-501 (1983)
© 1983 American Society of Plant Biologists

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Articles

Ammonia Assimilation in the Roots of Nitrate- and Ammonia-Grown Hordeum Vulgare (cv Golden Promise)

P. Anthony Fentem1, Peter J. Lea and George R. Stewart

Shell Research Ltd., Sittingbourne Research Centre, Sittingbourne, Kent ME98 8AG, United Kingdom, Department of Biochemistry, Rothamsted Experimental Station, Harpenden, Hertfordshire AL5 2JQ, United Kingdom, Department of Botany, Birkbeck College, University of London, London WC1E 7HX, United Kingdom

15N kinetic labeling studies were performed on seedlings of Hordeum vulgare L. var. Golden Promise growing under steady state conditions. Patterns of label incorporation in the pools of nitrogen compounds of roots fed [15N]ammonium were compared with computer-simulated labeling curves. The data were found to be quantitatively consistent with a three-compartment model in which ammonium is assimilated solely into the amide-N of glutamine. Labeling data from roots fed [15N]nitrate were also found to be at least qualitatively consistent with the assimilation of ammonia into glutamine. Methionine sulfoximine almost completely blocked the incorporation of 15N label into the amino acid pools of barley roots fed [15N]nitrate. These observations suggest that ammonia assimilation occurs solely via the glutamine synthetase/glutamate synthase pathway in both nitrate- and ammonia-grown barley roots.

1 SERC-CASE student in the Department of Botany, University of Manchester, and the Department of Biochemistry, Rothamsted, during the period of this work.




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