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Plant Physiology 70:1162-1168 (1982)
© 1982 American Society of Plant Biologists

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Fluorescence Immunohistochemical Localization of Malate Dehydrogenase Isoenzymes in Watermelon Cotyledons 1

A Developmental Study of Glyoxysomes and Mitochondria

Christof Sautter and Bertold Hock

Department of Botany, Faculty of Agriculture and Horticulture, Technical University of Munich, D-8050 Freising 12, West Germany

Monospecific antibodies to glyoxysomal, mitochondrial, and cytosolic I malate dehydrogenase were used for the fluorescence immunohistochemical localization of these isoenzymes in dark-grown watermelon (Citrullus vulgaris Schrad.) cotyledons. It was demonstrated that, with cell organelles isolated by sucrose density gradient centrifugation, antibodies to glyoxysomal malate dehydrogenase were specific markers for glyoxysomes, and similarly, antibodies to mitochondrial malate dehydrogenase were markers for mitochondria. The time course of the glyoxysomal malate dehydrogenase appearance and decline was not synchronous for the individual tissues and differed completely from that of the mitochondria. The cytosolic malate dehydrogenase I was confined to restricted regions of the lower epidermis. The activity which was definitively localized outside the cell organelles decreased during the first days of germination.

1 Supported by the Deutsche Forschungsgemeinschaft (Grant Ho 383/19).




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Copyright © 1982 by the American Society of Plant Biologists