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Plant Physiology 70:1075-1078 (1982)
© 1982 American Society of Plant Biologists

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Articles

Phospholipid Motional Characteristics in a Dry Biological System 1

A 31P-Nuclear Magnetic Resonance Study of Hydrating Typha latifolia Pollen

David A. Priestley2 and B. de Kruijff

Department of Plant Physiology, Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands, Institute of Molecular Biology, State University of Utrecht, Padualaan 8, P.O. Box 80.063, 3508 TB Utrecht, The Netherlands

Analysis of the proton-decoupled 31P-nuclear magnetic resonance (NMR) spectrum of fully hydrated Typha latifolia pollen revealed the presence of two main peaks: A broad asymmetrical component of a `bilayer' lineshape and a much narrower symmetrical component originating from phosphorus compounds undergoing rapid isotropic motion. From (a) 31P-NMR experiments on the hydrated total pollen phospholipids, (b) saturation transfer 31P-NMR experiments, and (c) the fraction of lipid phosphate in the pollen, it can be concluded that the great majority of the endogenous phospholipids are arranged in extended bilayers in which the lipid phosphates undergo fast ({tau}c < 10–6 second) long axis rotation. This bilayer arrangement of phospholipids was observed in the pollen down to hydration levels of at least 10.9% moisture content. At the lowest level of pollen hydration examined (5.2%) the 31P-NMR spectrum had a solid state lineshape demonstrating that all the phosphorus-containing compounds (including the phospholipids) were virtually immobile.

2 Present address: Boyce Thompson Institute, Cornell University, Ithaca, NY 14853.

1 Supported by a fellowship from the Netherlands Organization for the Advancement of Pure Research (Z. W. O.) to D. A. P.






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Copyright © 1982 by the American Society of Plant Biologists