Plant Physiol. Drug Metab Dispos
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Plant Physiology 70:232-237 (1982)
© 1982 American Society of Plant Biologists

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Evidence that Envelope and Thylakoid Membranes from Pea Chloroplasts Lack Glycoproteins 1

Kenneth Keegstra and Kenneth Cline

Department of Botany, University of Wisconsin, Madison, Wisconsin 53706

Envelope and thylakoid membranes from pea (Pisum sativum var. Laxton's Progress No. 9) chloroplasts were analyzed for the presence of glycoproteins using two different approaches. First, the sugar composition of delipidated membrane polypeptides was measured directly using gas chromatographic analysis. The virtual absence of sugars suggests that plastid membranes lack glycoproteins. Second, membrane polypeptides separated by sodium dodecyl sulfate gel electrophoresis were tested for reactivity toward three different lectins: Concanavalin A, Ricinus communis agglutinin, and wheat germ agglutinin. In each case, there was no reactivity between any of the lectins and the plastid polypeptides. Microsomal membranes from pea tissues were used as a positive control. Glycoproteins were readily detectable in microsomal membranes using either of the two techniques. From these results it was concluded that pea chloroplast membranes do not contain glycosylated polypeptides.

1 Supported in part by the United States Department of Agriculture Competitive Grant No. 5901-0410-9-0319-0 and by a National Institute of Health postdoctoral fellowship No. 1-F-32-GM7003-01 to K. C.






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Copyright © 1982 by the American Society of Plant Biologists