Plant Physiology 70:227-231 (1982)
© 1982 American Society of Plant Biologists
Articles
Isolation and Quantitation of  -D-Glucopyranosyl Abscisate from Leaves of Xanthium and Spinach 1,2
Gregory L. Boyer and
Jan A. D. Zeevaart3
Michigan State University—Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824
From previous work (Zeevaart 1980 Plant Physiol 66: 672-678) Xanthium leaves are known to contain a high level of alkali-hydrolyzable conjugated abscisic acid. This abscisic acid conjugate has been isolated and identified by mass spectrometry, nuclear magnetic resonance, and chemical and enzymic degradation techniques, as the glucosyl ester of abscisic acid,  -D-glucopyranosyl abscisate. The glucosyl ester of abscisic acid was the only abscisic acid conjugate found in Xanthium leaves. It was also isolated from spinach leaves.
An insignificant amount of the glucosyl ester of abscisic acid partitioned into diethyl ether, whereas 12% partitioned into ethyl acetate. Consequently, removal of abscisic acid by partitioning with ethyl acetate will result in considerable losses of the glucosyl ester of abscisic acid from the aqueous phase. Diethyl ether is, therefore, recommended for separation of abscisic acid and the glucosyl ester of abscisic acid by solvent partitioning.
A method for quantitation of the glucosyl ester of abscisic acid as the tetraacetate derivative by gas-liquid chromatography with an electron capture detector was developed. The level of -D-glycopyranosyl abscisate in Xanthium leaves increased from 3.6 nanomoles per gram fresh weight in turgid leaves to 22.9 nanomoles in leaves from plants subjected to seven wilting-recovery cycles. -D-glycopyranosyl abscisate in Xanthium leaves may be a stable end product of abscisic acid metabolism.
3 Author to whom correspondence should be addressed.
1 Supported by National Science Foundation Grant PCM 78-07653 and by the United States Department of Energy under Contract DE-ACO2-76ERO-1338.
2 The glucose ester of ABA can be named either as 1-O-abscisoyl--D-glucopyranose or -D-glucopyranosyl abscisate. To emphasize the fact that it is an ester of ABA and to avoid confusion with 1'-O-abscisic acid--D-glucopyranoside, we have chosen the latter nomenclature.
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