Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 69:1268-1273 (1982)
© 1982 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bamberger, E. S.
Right arrow Articles by Gibbs, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bamberger, E. S.
Right arrow Articles by Gibbs, M.
Agricola
Right arrow Articles by Bamberger, E. S.
Right arrow Articles by Gibbs, M.
Articles

H2 and CO2 Evolution by Anaerobically Adapted Chlamydomonas reinhardtii F-60 1

Elchanan S. Bamberger2, Dan King3, David L. Erbes4 and Martin Gibbs

Institute for Photobiology of Cells and Organelles, Brandeis University, Waltham, Massachusetts 02254

Using manometric and enzymic techniques, H2 and CO2 evolution in darkness and light has been studied in the green alga Chlamydomonas reinhardtii F-60. F-60 is a mutant strain characterized by an incomplete photosynthetic carbon reduction cycle but an intact electron transport chain.

In the dark, starch was broken down, and H2 and CO2 was released. The uncoupler, carbonyl cyanide m-fluorophenylhydrazone with an optimum concentration of 5 to 10 micromolar, increased the rate of CO2 release and starch breakdown but depressed H2 formation. It was suggested that carbonyl cyanide m-fluorophenylhydrazone increased the rate of starch breakdown by making the chloroplast membrane permeable to H+, removing a rate-limiting step, and leading to an altered fermentative pattern.

Photoevolution of H2 and CO2, but not starch breakdown, was stimulated by acetate. Maximum stimulation occurred at concentrations from 1 to 10 millimolar. Carbonyl cyanide m-fluorophenylhydrazone stimulated starch breakdown and CO2 and H2 release in the light, but not to the extent of acetate. Inasmuch as the uptake and subsequent metabolism of acetate required ATP, it was suggested that acetate, like carbonyl cyanide m-fluorophenylhydrazone, stimulated H2 photoproduction by removing ATP which limited the sequence of reactions. The contribution of photosystem II to the photoproduction of H2, as judged from the effect of 10 micromolar 3-(3,4-dichlorophenyl)-1, 1-dimethylurea, was at least 80%.

CO2 photoevolution increased linearly with time, but H2 photoevolution occurred in two phases: a rapid initial phase followed by a second slower phase. The rate of H2 release increased hyperbolically with light intensity, but the rate of CO2 production tended to level off and decrease with increasing light intensity, up to 145 watts per square meter. It was proposed that a changing CO2 and H2 ratio is the result of interaction between the carbon and hydrogen metabolism and the photosynthetic electron transport chain.

2 Present address: School of Education, University of Haifa, Oranim, Israel.

3 Present address: Biology Department, Lycoming College, Williamsport, PA 17701.

4 Biochemicals Department, E. I. DuPont de Nemours and Company, Wilmington, DE 19898.

1 Supported by Department of Energy (10-EY-76-5-02-3231) and National Science Foundation (PCM 79-22612).




This article has been cited by other articles:


Home page
 Eukaryot CellHome page
A. Hemschemeier, J. Jacobs, and T. Happe
Biochemical and Physiological Characterization of the Pyruvate Formate-Lyase Pfl1 of Chlamydomonas reinhardtii, a Typically Bacterial Enzyme in a Eukaryotic Alga
Eukaryot. Cell, March 1, 2008; 7(3): 518 - 526.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. C. Posewitz, S. L. Smolinski, S. Kanakagiri, A. Melis, M. Seibert, and M. L. Ghirardi
Hydrogen Photoproduction Is Attenuated by Disruption of an Isoamylase Gene in Chlamydomonas reinhardtii
PLANT CELL, August 1, 2004; 16(8): 2151 - 2163.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Melis and T. Happe
Hydrogen Production. Green Algae as a Source of Energy
Plant Physiology, November 1, 2001; 127(3): 740 - 748.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Florin, A. Tsokoglou, and T. Happe
A Novel Type of Iron Hydrogenase in the Green Alga Scenedesmus obliquus Is Linked to the Photosynthetic Electron Transport Chain
J. Biol. Chem., February 23, 2001; 276(9): 6125 - 6132.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1982 by the American Society of Plant Biologists