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Plant Physiology 69:1070-1076 (1982)
© 1982 American Society of Plant Biologists

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Methionine Biosynthesis in Lemna: Inhibitor Studies

Anne H. Datko and S. Harvey Mudd1

National Institute of Mental Health, Bethesda, Maryland 20205, Laboratory of General and Comparative Biochemistry, Bethesda, Maryland 20205

A search was made for compounds that would inhibit methionine biosynthesis in Lemna paucicostata Hegelm. 6746. DL-Propargylglycine (0.15 micromolar) produced growth inhibition and morphological changes which were prevented by exogenous methionine. Also, DL-propargylglycine inhibits cystathionine {gamma}-synthase activity. L-Aminoethoxyvinylglycine (0.05 micromolar) produced growth inhibition and morphological changes partially preventable by exogenous methionine. L-Aminoethoxyvinylglycine impairs the cleavage of cystathionine to homocysteine. Lysine and threonine, at concentrations which individually had little effect on growth or morphology of Lemna, together produced growth inhibition and morphological changes preventable by exogenous methionine. The resulting metabolic block prevented conversion of cysteine to cystathionine, presumably secondary to depletion of the supply of O-phosphohomoserine.

Inhibition of Lemna growth resulted when the molybdate:sulfate ratio in the medium was increased to 20:1 or more. Such inhibition was prevented by lowering this ratio to 0.3 or less. A non-steady-state experiment (molybdate:sulfate, 20:1) showed that molybdate inhibited sulfate uptake, but it provided no evidence of a further impairment in the organification of sulfate. Molybdate-induced growth inhibition of Lemna was prevented by cystine but not by cystathionine or methionine. Cystathionine is not converted by Lemna to cysteine rapidly enough to sustain growth.

1 To whom reprint requests should be addressed, at Building 32, Room 101, National Institute of Mental Health, Bethesda, MD 20205.




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