Plant Physiol. Drug Metab Dispos
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Plant Physiology 69:983-987 (1982)
© 1982 American Society of Plant Biologists

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Articles

Glutamine Synthetase in Spinach Leaves 1

IMMUNOLOGICAL STUDIES AND IMMUNOCYTOCHEMICAL LOCALIZATION

Bertrand Hirel2, Catherine Perrot-Rechenmann, Akira Suzuki, Jean Vidal and Pierre Gadal

Laboratoire de Biologie Végétale, Université de Nancy I, ERA au CNRS 799, C.O. 140, 54037 Nancy Cedex, France

By polyacrylamide gel electrophoresis, DEAE Sephacel, and hydroxyapatite chromatography, one form of glutamine synthetase has been identified in spinach (Spinacia oleracea L. cv. Monstrueux de Viroflay) leaves. It is localized only inside the chloroplast. The enzyme was purified to homogeneity and specific antibodies against the protein were raised by immunization of rabbits. The intracellular localization of glutamine synthetase in spinach leaves was studied by indirect immunofluorescence microscopy on thin-sectioned spinach leaves. It has been demonstrated that the enzyme is specifically associated with the chloroplasts of parenchymatous cells.

2 Present address: Laboratoire de Physiologie Végétale Métabolique, Université de Paris Sud, Centre d'Orsay, Bat 430, 91405 Orsay Cedex, France.

1 Supported by grant recherche cooperative sur programme 389 and by grant 78 70445 from the Délégation Générale à la Recherche Scientifique et Technique.






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Copyright © 1982 by the American Society of Plant Biologists