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Articles

Effect of Photoperiod on Metabolism of [³H] Gibberellins A₁, 3-epi-A₁, and A₂₀ in Agrostemma githago L. ¹

Department of Biochemistry, Michigan State University, East Lansing, Michigan 48824, MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824

To determine whether daylength influences the rate of metabolism of gibberellins (GAs) in the long-day (LD) rosette plant Agrostemma githago L., [³H]GA₂₀ and [³H]GA₁ were applied under short day (SD) and LD. Both were metabolized faster under LD than under SD. [³H]GA₂₀ was metabolized to a compound chromatographically identical to 3-epi-GA₁. [³H]GA₁ was metabolized to two acidic compounds, the major metabolite having chromatographic properties similar to, but not identical with GA₈. [³H]3-epi-GA₁ applied to plants under LD was metabolized much more slowly than was [³H]GA₁, and formed a very polar metabolite which did not partition into ethyl acetate at pH 2.5. Very polar metabolites were also formed after the feeds of [³H]GA₂₀ and [³H]GA₁. It was not possible to characterize these very polar compounds further because of their apparent instability. The results obtained suggest that in Agrostemma GA₂₀ is the precursor of 3-epi-GA₁, but there is at present no evidence indicating the precursor of GA₁.

³ To whom correspondence should be addressed.

¹ Supported by the United States Department of Energy Contract DE-AC02-76-ER01338.