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Isolation of a Wheat Cell Line with Altered Membrane Properties

Institute of Biophysics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary, Institute of Biochemistry, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary, Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary

A spontaneous dimethylsulfoxide (DMSO)-tolerant cell line was isolated from a cell culture of wheat (Triticum monococcum L.). The tolerant cells were able to grow in the presence of 4% DMSO. Cells formed from protoplasts of the tolerant line required DMSO for division in culture medium of high osmotic value.

Fatty acid composition and the molar ratio of phospholipids/sterols suggest a more ordered membrane structure in the tolerant line. Accordingly, a lower K⁺ influx rate was detected in the tolerant cells in comparison with the original line. These characteristics were maintained after 6 months' cultivation of the cells in DMSO-free growth medium. This suggested that genetic changes could be responsible for differences between the two cell lines.