Plant Physiol. Drug Metab Dispos
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Plant Physiology 69:456-459 (1982)
© 1982 American Society of Plant Biologists

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Articles

A Malic Acid Permease in Isolated Vacuoles of a Crassulacean Acid Metabolism Plant 1

Christa Buser-Suter, Andres Wiemken and Philippe Matile

Swiss Federal Institute of Technology, CH-8092 Zürich, Switzerland, Department of General Botany, CH-8092 Zürich, Switzerland

Vacuoles isolated from mesophyll protoplasts of the Crassulacean acid metabolism plant Bryophyllum daigremontianum take up [14C]L-malic acid from the medium. The initial rates of uptake show saturation kinetics (apparent Km about 1 millimolar). In competition experiments, the uptake rates of L-malic acid are greatly reduced in the presence of a surplus of D-malic and tartronic acid. A number of other acids are much less effective and aspartic and isocitric acid have virtually no effect. The uptake rate of malic acid is unchanged in the presence of ATP and the uncouplers dinitrophenol and carbonyl cyanide m-chlorophenyl hydrazone. When the vacuoles are isolated in the absence of bovine serum albumin or when 1 millimolar HgCl2 is added, they are inactive in transport but retain their malic acid pool. The existence of a specific permease which catalyzes an exchange diffusion of malic acid across the vacuolar membrane is postulated.

1 Supported by the Swiss National Science Foundation, Project No. 3.781.76 and by Burrus & Cie., Manufacture de Tabacs et Cigarettes, Boncourt, Switzerland.




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U. Lüttge, T. Pfeifer, E. Fischer-Schliebs, and R. Ratajczak
The Role of Vacuolar Malate-Transport Capacity in Crassulacean Acid Metabolism and Nitrate Nutrition. Higher Malate-Transport Capacity in Ice Plant after Crassulacean Acid Metabolism-Induction and in Tobacco under Nitrate Nutrition
Plant Physiology, November 1, 2000; 124(3): 1335 - 1348.
[Abstract] [Full Text]


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Copyright © 1982 by the American Society of Plant Biologists