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Inhibition of Ethylene Biosynthesis by Aminoethoxyvinylglycine and by Polyamines Shunts Label from 3,4-[¹⁴C]Methionine into Spermidine in Aged Orange Peel Discs ¹

Department of Horticulture, The Hebrew University of Jerusalem, Rehovot, Israel, Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, Israel

The flux of radioactivity from 3,4-[¹⁴C]methionine into S-adenosyl-L-methionine (SAM), 1-aminocyclopropane-1-carboxylic acid (ACC), spermine, and spermidine while inhibiting conversion of ACC to ethylene by 100 millimolar phosphate and 2 millimolar Co²⁺ was studied in aged peel discs of orange (Citrus sinensis L. Osbeck) fruit. Inhibition up to 80% of ethylene production by phosphate and cobalt was accompanied by a 3.3 times increase of label in ACC while the radioactivity in SAM was only slightly reduced. Aminoethoxyvinylglycine (AVG) increased the label in SAM by 61% and reduced it in ACC by 47%. Different combinations of standard solution, in which putrescine or spermidine were administered alone or with AVG, demonstrated clearly that inhibition of ethylene biosynthesis—at the conversion of SAM to ACC—by AVG, exogenous putrescine or exogenous spermidine, stimulated the incorporation of 3,4-[¹⁴C]methionine into spermidine.

³ Present address: Plant Hormone Laboratory, Room 205, Building 002, Beltsville. Agricultural Research Center (West), United States Department of Agriculture, Beltsville, MD 20705.

¹ Supported by a grant from the Israeli Ministry of Absorption to Z. E. C. and by a grant from the Israeli Ministry of Agriculture to R. G.

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