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¹⁵N- and [¹³C]NMR Determination of Utilization of Glycine for Synthesis of Storage Protein in the Presence of Glutamine in Developing Cotyledons of Soybean

Department of Biology, Washington University, St. Louis, Missouri 63130, Physical Sciences Center, Monsanto Co., St. Louis, Missouri 63166

Solid-state ¹⁵N- and [¹³C] NMR have been used to measure quantitatively the utilization of glycine in the presence of glutamine for the synthesis of storage protein in immature cotyledons of soybean (Glycine max L. cv. Elf) in culture. The presence of an equal molar amount of glycine in the medium causes a decrease in the use of glutamine-amide nitrogen. Glycine nitrogen is incorporated extensively into peptide bonds (in amounts greater than what would be expected if it appeared solely in glycine residues), but is used sparingly for synthesis of histidine ring residues, guanidino nitrogen residues of arginine, and lysine residues. The modest use of glycine carbon in protein synthesis does not parallel the use of glycine nitrogen.