Plant Physiol. Drug Metab Dispos
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Plant Physiology 68:851-853 (1981)
© 1981 American Society of Plant Biologists

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Articles

Effects of Leupeptin on Proteinase and Germination of Castor Beans 1

Amedeo Alpi2 and Harry Beevers

Biology Department, University of California, Santa Cruz, California 95064

Leupeptin, a tripeptide inhibitor of some proteinases, was shown previously to maintain the stability of several enzymes (isocitrate lyase, fumarase, and catalase) in crude extracts of castor bean endosperm. This reagent is now shown to inhibit the breakdown of water-soluble and crystalloidstorage proteins of the protein bodies isolated from castor beans by the SH-proteinase and it also inhibits the endopeptidase from mung beans. When suitably introduced into the endosperm of dry castor beans it strongly inhibits germination and seedling development. Application of leupeptin to endosperm halves removed from the seed prevents the normal development of enzymes concerned with gluconeogenesis from fat and drastically curtails sugar production. The results suggest that the SH-proteinase is intimately involved in the mobilization of storage proteins.

2 Recipient of a long-term fellowship from the European Molecular Biology Organization. Present address: Istituto di Orticultura, Universitá di Pisa, Viale delle Piagge 23, 56100 Pisa Italy.

1 Supported by Contract DE-AS03-76SF00034 from the United States Department of Energy.






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Copyright © 1981 by the American Society of Plant Biologists