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Plant Physiology 68:382-385 (1981)
© 1981 American Society of Plant Biologists

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Maintenance of Membrane Fluidity during Development of Freezing Tolerance of Winter Wheat Seedlings 1

M. Keith Pomeroy2 and John K. Raison

CSIRO Division of Food Research and School of Biological Sciences, Macquarie University, North Ryde, Sydney 2113, Australia, Plant Physiology Unit and School of Biological Sciences, Macquarie University, North Ryde, Sydney 2113, Australia

Fluidity of membrane lipids of shoot and root tissue and of chloroplasts from young wheat seedlings of contrasting freezing tolerance was investigated by measuring the motion and order parameters after spin labeling. A striking similarity was observed in membrane lipid fluidity of the five cultivars grown at 22 C. After cold hardening by growth at 2 C, a small change in membrane lipid fluidity was observed, but this was not correlated with the development of freezing tolerance, and there was no alteration in the transition temperature of membrane lipids. The results show that neither changes in membrane lipid fluidity nor transition temperature are a necessary feature of cold acclimation in wheat.


2 On leave from: Research Branch, Agriculture Canada, Ottawa, Canada, K1A 0C6. To whom reprint requests should be sent.

1 Contribution No. 1202 of the Chemistry and Biology Research Institute, Agriculture Canada.







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ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1981 by the American Society of Plant Biologists