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Plant Physiology 63:811-815 (1979)
© 1979 American Society of Plant Biologists

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Ethylene Production by Callus and Suspension Cells from Cortex Tissue of Postclimacteric Apples

Morris Lieberman, Shiow Y. Wang and Lowell D. Owens

Post Harvest Plant Physiology Laboratory and Cell Culture and Nitrogen Fixation Laboratory, Beltsville Agricultural Research Center, United States Department of Agriculture, Beltsville, Maryland 20705

Cortex tissue from postclimacteric `Golden Delicious' apples (Malus domestica, Borkh.) stored at 0 C for 9 months after harvest were induced to form callus in vitro. Cell suspension cultures were subsequently formed from calli. Of five media tested, only the medium of Schenk and Hildebrandt (Can J Bot 1972, 50: 192) and that of Uchimiya and Murashige (Plant Physiol 1974, 54: 936) allowed callus formation. During growth both the callus and cell cultures produced ethylene in a pattern which showed a rapid rise and then a fall as the culture grew. 14C-Labeled methionine was converted to labeled ethylene by the cell suspension cultures, which also could be inhibited from producing ethylene by a rhizobitoxine analog or free radical scavengers. Ethylene production in these cultures, like that in intact fruit tissue slices, could be stimulated by IAA or suppressed by N6-({gamma},{gamma}-dimethylallyl) adenosine and GA3.






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ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1979 by the American Society of Plant Biologists