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Plant Physiology 63:738-743 (1979)
© 1979 American Society of Plant Biologists

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Articles

Intracellular Localization of Some Key Enzymes of Crassulacean Acid Metabolism in Sedum praealtum1

Martin H. Spalding, Mark R. Schmitt, S. B. Ku and Gerald E. Edwards

a Department of Horticulture, University of Wisconsin, Madison, Wisconsin 53706

The intracellular locations of six key enzymes of Crassulacean acid metabolism were determined using enzymically isolated mesophyll protoplasts of Sedum praealtum D.C. Data from isopycnic sucrose density gradient centrifugation established the chloroplastic location of pyruvate Pi dikinase, the mitochondrial location of NAD-linked malic enzyme, and exclusively nonparticulate (not associated with chloroplasts, peroxisomes, or mitochondria) locations of phosphoenolpyruvate carboxylase, NADP-linked malic enzyme, enolase, and phosphoglycerate mutase. The consequences of this enzyme distribution with respect to compartmentalization of the pathway and the transport of metabolites in Crassulacean acid metabolism are discussed.

1 This work was supported by the College of Agriculture and Life Sciences, University of Wisconsin, Madison, by the University of Wisconsin Research Committee with funds from the Wisconsin Alumni Research Foundation, and by National Science Foundation Grant PCM 77-09384 to G. E. E.






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ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1979 by the American Society of Plant Biologists