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ATP Citrate Lyase from Germinating Castor Bean Endosperm

Localization and some Properties ¹

^a Thimann Laboratories, University of California, Santa Cruz, California 95064

ATP citrate lyase (EC 4.1.3.8) has been found in crude extracts from endosperm tissue of germinating castor bean and shows its maximum activity in 4- to 5-day-old seedlings. A strict requirement for coenzyme A and adenosine 5'-triphosphate was demonstrated. The pH optimum for the reaction is around 7.5. The unstable enzyme can be stabilized by freezing and addition of citrate and glycerol. (–)-Hydroxycitrate is a potent inhibitor. The molecular weight is about 400,000. The adenosine 5'-triphosphate citrate lyase is localized in the plastids, where it possibly plays a role in providing acetyl coenzyme A for lipid biosynthesis.

³ Present address: Biochemie der Pflanzen, Biologisches Institut II der Universität Freiburg, Schänzlestr. 9-11, 78 Freiburg, West Germany.

¹ Supported by DOE Contract EY-76-S-03-0034.

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