This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Owens, L. D. Search for Related Content PubMed PubMed Citation Articles by Owens, L. D. Agricola Articles by Owens, L. D.

Articles

Binding of ColE1-kan Plasmid DNA by Tobacco Protoplasts

Nonexpression of Plasmid Gene

United States Department of Agriculture, Science and Education Administration, Agricultural Research, Plant Physiology Institute, Beltsville, Maryland 20705

Protoplasts prepared from cultured tobacco cells were treated with ColE1-kan plasmid DNA, a hybrid of ColE1 and pSC105 plasmids bearing a gene for kanamycin resistance. The conditions employed permitted the uptake or irreversible binding of 2.9% of the added DNA in acid-insoluble form. Upon commencement of division, the treated cells were plated in agar medium containing kanamycin and differentiating hormones. Plantlets or shoots obtained as presumptive transformants were further tested on kanamycin medium by subculturing small leaf pieces. No evidence was obtained for expression of the kanamycin resistance gene of ColE1-kan in tobacco tissue.