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Amino Acid Levels in Carrot Cell Suspension Culture

No Correlation with Aspartate Kinase Isoenzyme Levels

Biochemistry Department, Rothamsted Experimental Station, Harpenden, Herts. AL5 2JQ, United Kingdom

Free and protein amino acids were analyzed during the growth cycle of a suspension culture of carrot (Daucus carota L.) in which there is a 10-fold increase in a lysine-sensitive isoenzyme of aspartate kinase during the early part of the growth cycle. There is little change in either total amino acids or in the amino acids derived from aspartate between 3 and 24 days of culture. It is estimated that the demand for net synthesis of aspartate-derived amino acids is decreased as the growth rate declines and that there is no correlation between the amount of extractable lysine-sensitive aspartate kinase and the amounts of amino acids being synthesized.