Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 62:522-525 (1978)
© 1978 American Society of Plant Biologists

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Articles

Modification of Disease Resistance of Tobacco Callus Tissues by Cytokinins 1

Geraldine T. Haberlach2, Allen D. Budde, Luis Sequeira and John P. Helgeson2

Department of Plant Pathology, University of Wisconsin, Madison, Wisconsin 53706

The effects of differing cytokinin and auxin concentrations on resistance of tobacco (Nicotiana tabacum L.) tissue cultures to race 0 of Phytophthora parasitica var. nicotianae were examined. With 1 micromolar kinetin and either 11.5 micromolar indoleacetic acid or 1 micromolar 2,4-dichlorophen-oxyacetic acid, tissues from resistant cultivars exhibited a "hypersensitive" reaction to zoospores of the fungus and subsequently were colonized only slightly. With susceptible cultivars or with tissues from resistant cultivars supplied with higher cytokinin levels (e.g. 10 micromolar kinetin), this hypersensitive reaction did not occur and tissues were heavily colonized. Benzylaminopurine and kinetin were particularly effective in eliminating both the hypersensitive reaction and disease resistance. Zeatin and 6-(3-methyl-2-butenylamino)purine were less effective. Increases in indoleacetic acid levels reversed the effects of high cytokinin concentrations. The balance of phytohormones apparently controls the host response to the fungus; thus, in this system, resistance or susceptibility can be studied without changing either host or fungal genotype.

2 Also, Plant Disease Resistance Research Unit, ARS, NCR, U.S. Department of Agriculture.

1 Supported in part by a specific cooperative agreement between the University of Wisconsin and the United States Department of Agriculture. Research cooperative with the College of Agricultural and Life Sciences, University of Wisconsin, Madison, and the Agricultural Research Service, United States Department of Agriculture.






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Copyright © 1978 by the American Society of Plant Biologists