Plant Physiology 62:44-48 (1978)
© 1978 American Society of Plant Biologists
Articles
Hydrolases in Vacuoles from Castor Bean Endosperm 1
Mikio Nishimura2 and
Harry Beevers
Thimann Laboratories, University of California, Santa Cruz, California 95064
Vacuoles were prepared from endosperm tissue of 4-day-old castor bean seedlings (Ricinus communis var. Hale) and purified on a stepped sucrose gradient. It was shown by assays of marker enzymes that there was only trace contamination of the final preparation by other organelles (mitochondria, glyoxysomes, nuclei, spherosomes, and plastids) and by cytoplasmic components. Hydrolytic enzymes (acid protease, carboxypeptidase, phosphodiesterase, RNAase, phytase and  -glucosidase) were present in the isolated vacuoles in amounts indicating a primarily vacuolar localization in vivo. The vacuoles also contained storage protein and high concentrations of sucrose. The over-all results indicate that the vacuoles from castor bean endosperm are the site of hydrolysis of the constituents of the protein bodies and are a temporary storage compartment for the sucrose produced from fat and protein reserves.
2 M. N. received a travel grant from The Japan Society for Promotion of Science and a grant from Matsunaga Foundation (Tokyo).
1 Supported by National Science Foundation Grant PCM 75-23566.
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