Plant Physiol.
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Plant Physiology 60:313-316 (1977)
© 1977 American Society of Plant Biologists

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Articles

Mesophyll Cell Protoplasts of Potato

Isolation, Proliferation, and Plant Regeneration 1

James F. Shepard and Roger E. Totten

a Department of Plant Pathology, Kansas State University, Manhattan, Kansas 66506

Mesophyll cell protoplasts were isolated from potato (Solanum tuberosum L. cv. Russet Burbank) leaves and induced to proliferate in culture. Protoplast division was observed only among preparations isolated from plants previously conditioned under short periods of low intensity illumination. Sustained growth and development of protoplast-derived calli (p-calli) occurred when they were maintained on defined media at 24 C under 500 lux lighting. Shoot bud development within p-calli was controlled by a number of factors including light, temperature, basic medium composition, nature and source of phytohormones, the continued presence of an osmoticum, low concentrations of a utilizable carbohydrate, and the developmental stage of the p-callus.

1 This work was supported by National Science Foundation Grants BMS74-14563 and PCM76-11007 and was largely completed at the Department of Plant Pathology, Montana State University, Bozeman, Montana 59715. Montana Agricultural Experiment Station Journal Series Paper No. 608.




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Copyright © 1977 by the American Society of Plant Biologists