Plant Physiology 60:286-289 (1977)
© 1977 American Society of Plant Biologists
Articles
Glucose Transport into Spinach Chloroplasts 1
Gisela Schäfera and
Ulrich Hebera
Hans W. Heldtb
a Botanisches Institut der Universität Düsseldorf, Germany,
b Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie der Universität München, Germany
The uptake of radioactively labeled hexoses and pentoses into the sorbitol-impermeable 3H2O space (the space surrounded by the inner envelope membrane) of spinach (Spinacia oleracea L.) chloroplasts has been studied using silicone layer filtering centrifugation. Of the compounds tested, D-xylose, D-mannose, L-arabinose, and D-glucose are transported most rapidly, followed by D-fructose and L-arabinose. The rate of L-glucose uptake is only about 5% of that of D-glucose.
The transport of D-glucose and D-fructose shows saturation characteristics, the Km for D-glucose was found to be about 20 mM. All sugars transport and phloretin inhibit D-glucose transport. The temperature dependency of D-glucose transport appears to have an activation energy of 17 kcal/mol.
With low external concentrations of D-glucose the transport into the chloroplasts proceeds until nearly the external concentration is reached inside the chloroplasts.
D-glucose transport is inhibited by high D-glucose concentrations in the medium. It is concluded that D-glucose and other hexoses are transported by carrier-mediated diffusion across the inner envelope membrane. This transport is similar to the transport of D-glucose into erythrocytes.
1 Part of these studies are included in a thesis by G. Schäfer (Mathematisch-Naturwissenschaftliche Fakultät der Universität Düsseldorf). This work has been supported by the Deutsche Forschungsgemeinschaft. The collaboration between the two laboratories was enabled by a NATO award.
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