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Strong Binding of Cytochrome c on the Envelope of Spinach Chloroplasts

¹ Laboratoire de Biologie Végétale, Département de Recherche Fondamentale, Centre d'Etudes Nucléaires et Université Scientifique et Médicale de Grenoble, 85 X 38041 Grenoble, France

Yeast cationic ferricytochrome c was able to bind to the spinach (Spinacia oleracea) chloroplast envelope with a low affinity (Kd = 1.1 µM). The total amount of low affinity binding sites was of the order of 50 nmol cytochrome c mg^–1 protein. We gave the evidence that binding of ferricytochrome c to the envelope was electrostatic and that the envelope membranes were strongly negatively charged. Addition of yeast ferricytochrome c to a preparation of intact washed chloroplasts (class I) induced a strong agglutination of chloroplasts.