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Plant Physiology 59:1098-1103 (1977)
© 1977 American Society of Plant Biologists

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Articles

Localization of Acid Hydrolases in Protoplasts

Examination of the Proposed Lysosomal Function of the Mature Vacuole 1

Henry C. Butcher2, George J. Wagner and Harold W. Siegelman

a Department of Biology, Brookhaven National Laboratory, Upton, New York 11973

The development of techniques to isolate and purify relatively large quantities of intact vacuoles from mature tissues permits direct biochemical analysis of this ubiquitous mature plant cell organelle. Vacuoles and a fraction enriched in soluble cytoplasmic constituents were quantitatively prepared from Hippeastrum flower petal protoplasts. Vacuolar lysate and soluble cytoplasmic fractions were examined for acid hydrolase activities commonly associated with animal lysosomes, and pH optima were determined. Esterase, protease, carboxypeptidase, {beta}-galactosidase, {alpha}-glycosidase and {beta}-glycosidase, not found in the vacuole lysate fraction, were components of the soluble cytoplasmic fraction. Acid phosphatase, RNase and DNase were present in both fractions. Vacuolar enzyme activities were also examined as a function of flower development from bud through senescent stages. The data obtained are not consistent with the concept that the mature plant cell vacuole functions as a generalized lysosome.

2 Present address: Dept. of Biology, Loyola College, 4501 North Charles St., Baltimore, Md. 21210.

1 Research carried out at Brookhaven National Laboratory under the auspices of the United States Energy Research and Development Administration and supported, in part, by Loyola College, Baltimore, Md. By acceptance of this article, the publisher and/or recipient acknowledges the United States Government's right to retain a nonexclusive, royalty-free license in and to any copyright concerning this paper.




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F. Barrieu and M. J. Chrispeels
Delivery of a Secreted Soluble Protein to the Vacuole via a Membrane Anchor
Plant Physiology, August 1, 1999; 120(4): 961 - 968.
[Abstract] [Full Text]


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ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1977 by the American Society of Plant Biologists