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Articles

Isolation of Uridine 5'-Pyrophosphate Glucuronic Acid Pyrophosphorylase and Its Assay Using ³²P-Pyrophosphate ¹

^a Department of Horticulture, University of Illinois, Urbana, Illinois 61801

A procedure was devised to detect and assay uridine 5'-pyrophosphate (UDP)-glucuronic acid pyrophosphorylase in plant extracts. Substrates are UDP-glucuronic acid and ³²P-pyrophosphate, and the ³²P-uridine 5'-triphosphate produced is selectively adsorbed to charcoal. The charcoal adsorption procedure is a modification of that used to determine ³²P-adenosine 5'-triphosphate produced by adenosine 5'-pyrophosphate glucose pyrophosphorylase, and the modification greatly improves the retention of uridine 5'-triphosphate.

³ Present address: Standard Fruit Co., La Ceiba, Honduras.

¹ This work was supported by Grants GB 8764 and PCM 74-19113 from the National Science Foundation and by the Illinois Agriculture Experiment Station.