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Plant Physiology 59:792-794 (1977)
© 1977 American Society of Plant Biologists

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Articles

Evaluation of a Chemical Method for Assay of Helminthosporium maydis Race T Toxin 1

Olen C. Yoder and Vernon E. Gracen, Jr.

a Departments of Plant Pathology and Plant Breeding, Cornell University, Ithaca, New York 14853

A chemical test reported by Karr et al. (Plant Physiol. 55:727) to assay for host-specific toxin produced by Helminthosporium maydis race T was evaluated. Preparations from culture filtrates of both race T isolates, containing host-specific toxin, and similar preparations from race O isolates, containing no detectable host-specific toxin, gave positive reactions in the chemical assay. Also, preparations containing active or inactive toxin gave equal responses in this test. The procedure does not provide a reliable method for assaying H. maydis race T toxin.

1 This work was supported by United States Department of Agriculture C.S.R.S. Grant 216-15-24 and by Rockefeller Foundation Grants 7422 and 75002.






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Copyright © 1977 by the American Society of Plant Biologists