This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Saunders, J. A. Articles by Stocking, C. R. Search for Related Content PubMed PubMed Citation Articles by Saunders, J. A. Articles by Stocking, C. R. Agricola Articles by Saunders, J. A. Articles by Stocking, C. R.

Articles

Subcellular Localization of the Cyanogenic Glucoside of Sorghum by Autoradiography ¹

Chin Ho Lin^b,2 and C. Ralph Stocking^b

^a Department of Biochemistry and Biophysics, Department of Botany, University of California, Davis, California 95616

The use of microautoradiography at the electron microscopic level indicates that the vacuole is the site of accumulation of the cyanogenic glucoside of Sorghum bicolor. When a specific biosynthetic precursor of dhurrin, p-hydroxy[3,5-³H]phenylacetaldoxime, was used, 90% of the tritium label was recovered in the vacuoles of tissue prepared for microautoradiography. L-[3,5-³H]Tyrosine and D-[1-³H(N)]glucose, nonspecific precursors of dhurrin, of differing solubilities and biosynthetic capacity, were also fed to the shoots. The data obtained from these controls indicated that the high recovery of label in the vacuole of aldoxime-fed shoots was not indicative simply of the size of the vacuole, nor was it a result of movement of labeled compounds during preparation of the tissue for electron microscopy. The problem of movement of these labeled compounds during dehydration of tissue was dramatically reduced by chemical dehydration in 2,2-dimethoxypropane in less than 30 minutes rather than with routine dehydration in acetone or alcohol series for 24 hours.

¹ This work was supported in part by National Science Foundation Grants BMS 72-02275-A02 to C. R. S., PCM 74-11997-AO1 to E. E. C., and United States Public Health Service Grant GM 05301-19 to E. E. C.

This article has been cited by other articles:

				A. V. Morant, N. Bjarnholt, M. E. Kragh, C. H. Kjaergaard, K. Jorgensen, S. M. Paquette, M. Piotrowski, A. Imberty, C. E. Olsen, B. L. Moller, et al. The {beta}-Glucosidases Responsible for Bioactivation of Hydroxynitrile Glucosides in Lotus japonicus Plant Physiology, July 1, 2008; 147(3): 1072 - 1091. [Abstract] [Full Text] [PDF]