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Plant Physiology 58:717-718 (1976)
© 1976 American Society of Plant Biologists

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Articles

pH Changes in the Cytoplasm of the Blue-Green Alga Anacystis nidulans Caused by Light-dependent Proton Flux into the Thylakoid Space 1

Gernot Falknera and Franz Hornera

Karl Werdanb and Hans W. Heldtb

a Limnologisches Institut der Österreichischen Akademie der Wissenschaften, b Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie der Universität München

The pH in the cytoplasmic and thylakoid spaces of the blue-green alga, Anacystis nidulans, has been determined in the light and in the dark by uptake of 5,5-dimethyloxazolidine-2,4-dione and methylamine into the sucrose-impermeable 3H-H2O space, as measured by silicon layer filtering centrifugation.

Illumination causes an alkalinization in the cytoplasm which is accompanied by an acidification in the thylakoid space, reflecting light-dependent proton transport across the thylakoid membrane. Under light conditions, a pH gradient of approximately 2.8 between the cytoplasmic and thylakoid spaces has been measured that can be abolished almost completely by addition of the uncoupler, 3-chlorocarbonyl cyanide phenylhydrazone. The pH in the cytoplasm is independent of the pH in the medium.

1 This work has been supported by a grant from the Österreichischer Forschungsförderungsfonds and the Deutsche Forschungsgemeinschaft.




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Copyright © 1976 by the American Society of Plant Biologists