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Plant Physiology 58:675-680 (1976)
© 1976 American Society of Plant Biologists

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Articles

Membrane-bound UDP-Glucose

Lipid Glucosyltransferases from Peas 1

Rafael Pont Lezicaa,2

Pedro A. Romerob,3 and Marcelo A. Dankertb

a Departamento de Biología, Fundación Bariloche, C.C. 138, 8400 San Carlos de Bariloche, Río Negro, Argentina, Instituto de Investigaciones Bioquímicas "Fundación Campomar" and Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina

An enzymic preparation from peas (Pisum satisum), able to form neutral and polar glycosides, is described. The best sugar donor is UDP-glucose and the acceptors are present in the enzymic system.

The neutral glycolipids have been characterized as steryl and acylated steryl glucosides. The polar glucolipid had been identified as polyprenyl monophosphate glucose. The glucose is linked to the phosphate in beta configuration. Polar glucolipids are also formed from UDP-glucose and exogenous prenylic acceptors, either {alpha}-saturated, as dolichyl monophosphate, or allylic, as ficaprenyl monophosphate.

The two glucosylating activities have been partially separated by differential centrifugation: the fraction that precipitates at 25,000g has most of the neutral glucolipid-synthesizing activity, and the fraction that sediments at 100,000g is rich in polar glucolipid glucosyl transferase activity. This latter activity was strongly dependent on Mg2+ concentration, the optimum being around 5 to 10 mM. UDP inhibits the reaction and 0.2 to 0.5% (v/v) Triton X-100 has a stimulatory effect. The optimum pH is 7.5.

2 Career Investigator of the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.

3 Holder of an O.A.S. fellowship while on leave from the Universidad Católica de Valparaiso, Chile.

1 This work was supported in part by grants from the National Institutes of Health (U. S. A.) (No. GM 19808-02), the Universidad de Buenos Aires, the Consejo Nacional de Investigaciones Científicas y Técnicas, and the Programa Regional de Desarrolo Científico y Tecnológico of O.A.S.






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Copyright © 1976 by the American Society of Plant Biologists