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Dormancy in Seeds of Charlock (Sinapis arvensis L.)

Early Effects of Gibberellic Acid on the Synthesis of Amino Acids and Proteins ¹

^a Department of Physiology and Environmental Studies, School of Agriculture, University of Nottingham, Sutton Bonington, Loughborough, LE12 5RD, England

Charlock (Sinapis arvensis L.) seeds were imbibed with 10 mM GA₃ for 24 hours at 0 C. After equilibration at 25 C, a 5-fold increase in radioactivity in the amino acids labeled from 2-¹⁴C-acetate was observed within 2 hours. The total amount of amino acids was reduced to half, and the specific radioactivity increased approximately 10-fold, indicating a diversion of metabolites for amino acid and protein synthesis in GA₃-treated seeds. The rate of incorporation of L-¹⁴ C-leucine into protein was doubled. Autoradiographs showed that enhancement of protein synthesis was localized in the shoot and root meristems, the developing vascular tissues, and in the endosperm cells inside the testa.