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Plant Physiology 58:530-536 (1976)
© 1976 American Society of Plant Biologists

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Articles

Lettuce Seed Germination

Modulation of Pregermination Protein Synthesis by Gibberellic Acid, Abscisic Acid, and Cytokinin 1

David W. Fountain2 and J. Derek Bewley

a Department of Biology, University of Calgary, Calgary, Alberta, Canada T2N 1N4

Protein synthesis in gibberellin-treated lettuce (Lactuca sativa) seeds has been studied during the lag phase between the beginning of imbibition and the first signs of radicle protrusion. When compared to the water-imbibed controls, both polyribosome populations and radioactive leucine incorporation into protein increase in the embryos of GA3- induced seeds early in the imbibition period. Since these results are contradictory to previously published studies, the reasons for the differences are outlined and various alternative possibilities eliminated. The protocol for protein extraction, particularly the speed at which the supernatant from the seed homogenate is cleared, is important for demonstrating the GA3-mediated changes. Embryos maintained in the dormant state by abscisic acid still conduct considerable amounts of protein synthesis, and this is enhanced by concentrations of 6-benzylaminopurine which also promote germination. Therefore, the actions of GA3, abscisic acid, and cytokinin on lettuce seed germination are mediated, directly or indirectly, via protein synthesis.

2 Work completed as part of a Ph.D. thesis at The University of Calgary while the holder of a National Research Council of Canada postgraduate fellowship. Present address: Biology Division, Oak Ridge National Laboratory, P.O. Box Y, Oak Ridge, Tenn. 37830.

1 This work was supported by National Research Council of Canada Grants A6352 and E2550 to J. D. B. and an appropriation from The University of Calgary.






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Copyright © 1976 by the American Society of Plant Biologists