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Plant Physiology 57:304-307 (1976)
© 1976 American Society of Plant Biologists

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Estimated Drainage of Carbon from the Tricarboxylic Acid Cycle for Protein Synthesis in Suspension Cultures of Paul's Scarlet Rose Cells

Larry Hunt and John S. Fletcher

Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73069

The amount of carbon (µmoles of carbon atoms) drained from the tricarboxylic acid cycle for protein synthesis was compared with µmoles of CO2 released from the cycle at 2-day intervals during the growth of suspension cultures of Paul's Scarlet rose. We concluded that during the period of most rapid protein synthesis (day 0-4) one-sixth as much carbon was drained from the tricarboxylic acid cycle for protein synthesis as was released as CO2. By day 8, one-thirtieth of the amount of carbon released as CO2 was incorporated into protein. Net protein synthesis stopped on day 8, but the evolution of CO2/culture continued at its maximum rate until day 10.

Similar ratios were calculated based on the recovery of 14C in protein versus CO2 following a 3-hr provision of labeled substrates to 3-day-old cells (age of maximum protein synthesis). Provision of acetate-1-14C and acetate-2-14C indicated from one-eighth to an equal amount of carbon was incorporated into protein as was released as CO2. When 14C-labeled intermediates of the tricarboxylic acid cycle were provided, the ratio of 14C incorporated into protein versus that evolved in CO2 ranged from 1/0.9 to 1/4.9.

Following a critical analysis of the methods used, it was concluded that during periods of rapid protein synthesis, a conservative estimate of the amount of carbon drained from the tricarboxylic acid cycle for protein synthesis was one-fourth of the amount evolved as CO2 from the cycle.






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Copyright © 1976 by the American Society of Plant Biologists