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Articles

Endogenous Levels of Auxin and Tumorigenesis in a Nicotiana Amphiploid ^1,2

^a Department of Anatomy, State University of New York, Upstate Medical Center, Syracuse, New York 13210

Young seedlings of the tumor-prone amphiploid Nicotiana suaveolens X N. langsdorffii were grown aseptically on nutrient medium in a controlled environment chamber. At regular intervals after the seeds had been sown, the incidence of tumor formation was scored and plants were harvested. Indole-3-acetic acid was extracted, purified, and assayed spectrofluorometrically. A close correlation in time between onset of tumor formation and decline in endogenous level of indole-3-acetic acid was demonstrated.

² Presented at the Annual Meeting of the American Society of Plant Physiologists, Cornell University, Ithaca, N.Y. June 19, 1974.