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Concentrations of Indole-3-acetic Acid and Its Esters in Avena and Zea^1,2

^a Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan 48824

An isotope-dilution method has been developed for the assay of free indole-3-acetic acid and ester indole-3-acetic acid as measured by indole-3-acetic acid liberated by mild alkaline hydrolysis. Application of this method to seedlings of Avena sativa and Zea mays indicates the upper limit of free indole-3-acetic acid in Avena to be about 16 µg per kg and in Zea, about 24 µg. The amount of 1 N alkali-labile indole-3-acetic acid in Zea is about 330 µg per kg and there is very little 1 N alkali-labile IAA in Avena. A chemical characterization of the indole-3-acetic acid of Avena and a confirmation of the chemical characterization of the indole-3-acetic acid of Zea is presented.

² Dedicated to Solon A. Gordon.

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