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Plant Physiology 53:258-260 (1974)
© 1974 American Society of Plant Biologists

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Articles

Rapid Estimates of Relative Water Content

Richard E. Smarta,1

Gail E. Binghamb,2

a Department of Agriculture, Viticultural Research Station, Griffith, New South Wales, Australia, Department of Agronomy, Cornell University, Ithaca, New York 14850

Relative water content may be accurately estimated using the ratio of tissue fresh weight to tissue turgid weight, termed here relative tissue weight. That relative water content and relative tissue weight are linearly related is demonstrated algebraically. The mean value of r2 for grapevine (Vitis vinifera L. cv. Shiraz) leaf tissue over eight separate sampling occasions was 0.993. Similarly high values were obtained for maize (Zea mays cv. Cornell M-3) (0.998) and apple (Malus sylvestris cv. Northern Spy) (0.997) using a range of leaf ages. The proposal by Downey and Miller (1971. Rapid measurements of relative turgidity in maize (Zea mays L.). New Phytol. 70: 555-560) that relative water content in maize may be estimated from water uptake was also investigated for grapevine leaves; this was found to be a less reliable estimate than that obtained with relative tissue weight. With either method, there is a need for calibration, although this could be achieved for relative tissue weight at least with only a few subsamples.

1 Present address: Department of Pomology, Cornell University, Ithaca, N.Y. 14850.

2 Present address: 6204A Baker Circle, Edgewood, Maryland, 21040.






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Copyright © 1974 by the American Society of Plant Biologists