Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 51:807-809 (1973)
© 1973 American Society of Plant Biologists

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Use of Dextran-40 Gradients for Separation of Pea Cotyledon Mitochondria into Different Fractions 1

T. Solomos2, S. S. Malhotra and Mary Spencer

a Department of Plant Science, University of Alberta, Edmonton, Alberta, Canada

A crude pea (Pisum sativum L. var. Homesteader) mitochrondrial preparation was divided into two equal parts. One part was layered on a Dextran-40 step gradient, and the other on a sucrose step gradient, and they were centrifuged to obtain different bands of particles. The densities at which the particles banded and the mitochondrial respiratory activities of the particles were determined. Dextran-40 density gradient centrifugation resulted in a better separation of mitochondrial populations than did sucrose density gradient centrifugation. Separation by sucrose density gradient centrifugation may not be according to the true densities of the particles. On the other hand, the use of gradients of Dextran-40, a solute of low osmotic potential, facilitated separation of particles acording to their true densities. Such mitochondria showed better respiratory control ratio and ADP:0 values, than those isolated by sucrose density gradient centrifugation.

2 Present address: Department of Botanical Sciences, University of California, Los Angeles, Calif. 90024.

1 Supported by Grant A 1451 from the National Research Council of Canada, and a University of Alberta Post-Doctoral Fellowship to T. Solomos.






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Copyright © 1973 by the American Society of Plant Biologists