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Protein Synthesis by Isolated Etioplasts and Chloroplasts from Pea and Wheat and the Effects of Chloramphenicol and Cycloheximide ¹

Biology Division, Oak Ridge National Laboratory, The University of Tennessee-Oak Ridge Graduate School of Biomedical Sciences, Oak Ridge, Tennessee 37830, The Plant Physiology Unit, Commonwealth Scientific and Industrial Research Organization Division of Food Preservation, Ryde, and School of Biological Sciences, The University of Sydney, Sydney 2006, Australia.

Etioplasts capable of incorporating ¹⁴C-leucine into protein have been isolated from dark-grown pea and wheat plants. The requirements for leucine incorporation for etioplasts were similar to those for chloroplasts. An ATP-generating system, Mg²⁺, and GTP were required. The amino-acid-incorporation activity of etioplasts from wheat was comparable to that of chloroplasts on an RNA basis, whereas the activity of pea etioplasts was about 50% of the activity of pea chloroplasts. The incorporation of leucine into protein by etioplasts and chloroplasts from pea and wheat was inhibited by chloramphenicol, and to a slight extent by cycloheximide.

³ Fulbright-Hayes Fellow and Visiting Professor at the University of Tennessee, Oak Ridge Graduate School of Biomedical Sciences.

⁴ Present address: Department of Biochemistry, University of Massachusetts, Amherst, Mass.

¹ Research was jointly sponsored by the National Institute of General Medical Sciences, National Institutes of Health, Grant GM 15595, and by the United States Atomic Energy Commission under contract with the Union Carbide Corporation.