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Plant Physiology 50:176-182 (1972)
© 1972 American Society of Plant Biologists

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Articles

Purification and Partial Characterization of a Glucan Containing Indole-3-acetic Acid 1

Zdzislaw Piskornik2 and Robert S. Bandurski

a Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan 48823

The "bound auxin" of Zea mays, first described by Berger and Avery (Amer. J. Bot. 1944; 31: 199-203) has been purified and partially characterized. It is an indole-3-acetic acid-containing, high molecular weight, lipophilic cellulosicglucan. The indole-3-acetic acid is in ester linkage as evidenced by indoleacetamide formation upon ammonolysis. The glucan is of variable chain length and comprises, in general, 35 to 50 per cent of the dry weight of the compound. The glucosidic residues are {beta} 1 -> 4 linked and are hydrolyzed by cellulase. Mild acid hydrolysis produces cellobiose and cellotriose. Other components, as yet unidentified, of the compound are described.


2 On leave from the Agricultural University, Cracow, Poland, as an exchange fellow of the Polish-American Agricultural Exchange Program.

1 This work was supported, in part, by the National Science Foundation Grant GB-18353-X. Journal Article No. 5974 from the Michigan Agricultural Experiment Station.




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