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Characterization of Short Time Labeled Adenosine Monophosphate-rich Ribonucleic Acids of Soybean ¹

^a Department of Botany, University of Georgia, Athens, Georgia 30601

The total population of newly synthesized ³²P-AMP-rich RNA has been separated into two major types based on repeated fractionation on methylated albumin-kieselguhr columns. The purified D-RNA which elutes, under our experimental conditions, primarily in the salt gradient has a GMP/AMP ratio of about 0.8 and an AMP + UMP content of about 56 mole per cent. The purified TB-RNA which preferentially remains bound to the column in the salt gradient has a GMP/AMP ratio of about 0.4 to 0.45 and an AMP + UMP content of about 65 mole per cent. In addition to being distinguished by their fractionation on the methylated albumin-kieselguhr column and base composition analysis, purified D-RNA and TB-RNA have different size distributions on sucrose gradient and acrylamide gel fractionation, are differentially associated with polyribosomes and have different stabilities in the tissue.

³ Present address: Department of Biology, University of Houston, Houston, Texas 77004.

¹ This work was supported by Atomic Energy Commission Contract AT-(38-1)-643.