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Articles

Terminal Oxidases of Chlorella pyrenoidosa^1,2

^a Department of Biophysics, University of Western Ontario, London, Canada

In studies of the kinetics of oxygen uptake by glucose-stimulated Chlorella pyrenoidosa, two terminal oxidases could be distinguished. The cytochrome oxidase of Chlorella has a Km (O₂) of 2.1 ± 0.3 µM, while the second oxidase has a Km (O₂) of 6.7 ± 0.5 µM, and a maximum capacity about one-quarter of that of the cytochrome system. The identity of the second oxidase is unknown, but it is not inhibited by carbon monoxide, 1 mM cyanide, 0.1 mM thiocyanate, or 1 mM 8-hydroxyquinoline. In fresh cultures, the second oxidase accounts for at most 35% of the total oxygen uptake.

⁴ Reprint requests should be sent to this author, at the University of Western Ontario.

¹ This research was supported by the National Research Council of Canada Grant A6230.

² The work reported in this paper is part of the thesis submitted by D. F. Sargent in partial fulfillment of the requirements for the degree of Ph.D., University of Western Ontario, August 1971.