This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Klein, S. Articles by Schiff, J. A. Search for Related Content PubMed PubMed Citation Articles by Klein, S. Articles by Schiff, J. A. Agricola Articles by Klein, S. Articles by Schiff, J. A.

Articles

The Correlated Appearance of Prolamellar Bodies, Protochlorophyll(ide) Species, and the Shibata Shift during Development of Bean Etioplasts in the Dark ¹

^a Department of Biology, Brandeis University, Waltham, Massachusetts 02154

The structure and physiology of the etioplast was investigated in developing primary leaves of 3- to 9-day-old dark-grown bean (Phaseolus vulgaris L. var. Red Kidney) seedlings. Increase in total protochlorophyll(ide) content followed that of leaf fresh weight. In 3- to 4-day-old bean leaves more than 50% of the protochlorophyll(ide) is in the form of protochlorophyll(ide) 628, which is nontransformable by light. Most of the transformable pigment is protochlorophyll(ide) 635, with smaller amounts of protochlorophyll(ide) 650. During leaf development from the 3rd to the 7th day phototransformable protochlorophyll(ide) with an absorbance maximum at 650 nm accumulates faster than nontransformable protochlorophyll(ide) or protochlorophyll(ide) 635. This increase in protochlorophyll(ide) 650 is correlated with the formation and enlargement of prolamellar bodies.

The transformable protochlorophyll(ide) is converted by light directly to chlorophyll(ide) 672 in young leaves which do not yet have prolamellar bodies, and chlorophyll(ide) 672 may arise largely from the protochlorophyll(ide) 635. In older leaves the protochlorophyll(ide), largely protochlorophyll(ide) 650, is converted to chlorophyll(ide) 683, and a Shibata shift results in a change in the wavelength of absorption to 672 nm. The increase in protochlorophyll(ide) 650, the formation of prolamellar bodies, and the presence of the Shibata shift appear to be closely correlated. A model is briefly presented to provide a unified interpretation of these findings, including certain similarities between dark-grown Euglena cells and 3- to 4-day-old etiolated bean leaves.

¹ Research was supported by Grants GM14595 and RR 7044 from the National Institutes of Health.

This article has been cited by other articles:

				A. F. Abdelkader, H. Aronsson, K. Solymosi, B. Boddi, and C. Sundqvist High salt stress induces swollen prothylakoids in dark-grown wheat and alters both prolamellar body transformation and reformation after irradiation J. Exp. Bot., July 1, 2007; 58(10): 2553 - 2564. [Abstract] [Full Text] [PDF]

				U. Sperling, F. Franck, B. van Cleve, G. Frick, K. Apel, and G. A. Armstrong Etioplast Differentiation in Arabidopsis: Both PORA and PORB Restore the Prolamellar Body and Photoactive Protochlorophyllide -F655 to the cop1 Photomorphogenic Mutant PLANT CELL, February 1, 1998; 10(2): 283 - 296. [Abstract] [Full Text] [PDF]